A simple and Comprihensive ppt. Did you find this document useful? Is this content inappropriate? Report this Document. Description: High Performance Liquid Charomatography. Flag for inappropriate content. Download now. Related titles. Carousel Previous Carousel Next. Capillary Electrophoresis for the Analysis of Small-molecule Pharmaceuticals. Jump to Page. Search inside document. Mihaela Dragota. Jonathan Mares. Drkrishnasarma pathy. Bhargav Yeluri. Nikita Singh.
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Rekha Singh. Carlos Salvatierra Gil. Popular in Biology. On the other hand, the PDA detector adds a third dimension wavelength, which is a more convenient way of finding out the wavelength without having the need to repeat the analysis. In chromatography, the RF value pertains to the distance a particular component traveled divided by the distance traveled by the solvent front.
In other words, it is the characteristic of the component, which is helpful in the identification of the components. There are different types of chromatography but the two primary types are liquid chromatography and gas chromatography. Then searching related articles with specified keywords on internet search engines like Google Scholar or databases like PUBMED will help you out a lot for choosing the topic. I wish you a very good luck. Written in simple way… easily understandable language… good work… thanks DRubaa.
Hello sir, I want to know about hplc. Ye test kaise hota hai. Actually ye test Dr. Ne mere bete ko prescribe kiya hai. Hi Sir!!! Could we please establish a collaboration? Your email address will not be published. Save my name, email, and website in this browser for the next time I comment.
Skip to content. Table of Contents. What is the HPLC principle? The principle of HPLC is based on analyte distribution between the mobile and stationary phases. What are the types of HPLC? There are four primary types of HPLC — 1. Normal phase HPLC effective method for separating phospholipid classes 2. Reverse phase HPLC the most common method used to separate compounds that have hydrophobic moieties 3.
What are the 4 types of chromatography? The four types of chromatography are 1. Liquid chromatography test for pollution in water samples like lakes and rivers 2.
Gas chromatography detect bombs and useful in forensic investigations 3. Thin-layer chromatography used to check the purity of organic compounds such as the presence of insecticide or pesticide in foods 4. Paper chromatography uses a strip of paper in the stationary phase.
Why is high pressure needed in HPLC? What is the difference between isocratic and gradient? Your application can be run in different ways — isocratic and gradient. What solvent is used in HPLC? What are the advantages of HPLC? What is Rf value? What are the two main types of chromatography? Similar Posts. Thank you for information…. It helps me for my presentation. Sir it is very useful for me and anybody can understand easily from this presentation.
The information that is available in this presentation is better for me. Explanation is easily understood and straight to the point. Very helpful! Thank you! Want to know that, what pore size column we use for synthetic tripeptides in hplc. Very Helpful.. Hplc or GC ka ki full knowledge Hindi me kese pata kru frnz anyone help me plz…. Leave a Reply Cancel reply Your email address will not be published. Home Biochemistry Microbiology Expand child menu Expand.
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